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| Menezes,Caroline Anunciação; Gonçalves,Danielle Silva; Amianti,Jackeline; Fernandes,Irene; Taddei,Carla Romano; Koga,Paula Célia Mariko; Trabulsi,Luiz Rachid; Martinez,Marina Baquerizo; Piazza,Roxane Maria Fontes. |
| A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Detection; Polyclonal antibody; Monoclonal antibody; Thermo-labile toxin; E. coli. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500004 |
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| Almeida,Margarete Teresa Gottardo; Rubio,Fernando Gôngora; Garcia,Doroti Oliveira; Pavarino-Bertelli,Érika Cristina; Rossit,Andrea Regina Baptista; Bando,Silvia Yano; Silbert,Suzane; Goloni-Bertollo,Eny Maria; Soares,Márcia Maria Costa Nunes; Martinez,Marina Baquerizo. |
| Stenotrophomonas maltophilia is a Gram-negative bacillus, which is becoming widely recognized as an important nosocomial pathogen. The main objective of this study was to evaluate the genetic relatedness, by random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of 86 clinical isolates of S. maltophilia (colonization 22, infection 64) obtained from 79 hospitalized patients, from different geographic regions of São Paulo State. The genotypic analysis performed by RAPD and PFGE was used in 24 isolates for genetic identity confirmation. The results were congruent between the two methods but it was not possible to link genetic profiles with the studied variables, clinical state and geographic area, probably due to the great... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stenotrophomonas maltophilia; RAPD; PFGE. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200017 |
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| Garcia,Doroti de Oliveira; Timenetsky,Jorge; Martinez,Marina Baquerizo; Francisco,Waldemar; Sinto,Sumiko I.; Yanaguita,Roberto Mitio. |
| Forty-six S. maltophilia isolates obtained from hospital clinical specimens were studied for protease (caseinase and elastase) production, hemolytic activity, adhesion to HEp-2 cells, plastic and glass. Susceptibility to antimicrobial agents was also evaluated. The majority of isolates were obtained from respiratory tract secretions of patients using medical devices. All the isolates grown overnight were able to hydrolyze casein at 30ºC and 37ºC. After 72h, all the isolates hydrolyzed elastase at 30ºC and 40 isolates (87%) at 37ºC. Most of the isolates presented hemolytic activity after 96h of incubation at both temperatures. Rabbit blood showed the hightest hemolytic activity, after 96h 61% and 98% of tested isolates presented beta-hemolysis at 30ºC and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stenotrophomonas maltophilia proteases; Elastase hemolysins adhesion antimicrobial agents. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200012 |
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| Munford,Veridiana; Souza,Eloisa Correia; Caruzo,Thabata Alessandra Ramos; Martinez,Marina Baquerizo; Rácz,Maria Lúcia. |
| From a total of 187 fecal samples from children with ages between 0 and 5 years, collected in the Hospital Universitário -USP, Brazil, from 1994 to 1996, 54 (28.9%) were positive for rotavirus. Positive samples were characterized by electropherotyping, subgrouping, G serotype and genotype and P genotype. Rotavirus electropherotypes were characterized in four different long genome patterns (38.9%), one short genome pattern (34.0%) and 18.0% were characterized as an unusual pattern. Subgroup I was found in 38.9% strains, subgroup II in 50.0% and 7.7% was subgroup nonI-nonII. For G serotypes, G2 was found in 59.3%, G1 was identified in 33.3% of strains, two samples showed mixtures of G1+G2 and one sample was G1+G3. Ten samples characterized as serotype G2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Gastroenteritis; PCR; Serotypes; Genotypes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300014 |
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| Dall´Agnol,Monique; Martinez,Marina Baquerizo. |
| This work aimed the study of acquisition of iron from different compounds by EIEC strains. Seven serotypes were studied (O28ac.H-, O29:H-, O136:H-, O144:H-, O152:H-, O164:H- and O167:H7-), in a total of 31 strains. All serotypes were able to use distinct iron sources: hemin, hematin, hemoglobin, ovotransferin, lactoferrin, transferrin and ferritin. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: EIEC; Iron; Iron uptake. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200012 |
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